Abstract
Plastins are a family of at least three cytoplasmic protein isoforms that are expressed differentially between cells of the hematopoietic lineages and cells of solid tissues. Expression of the L-plastin isoform appears to be restricted to replicating blood cells, and the two T-plastin isoforms appear to be restricted to replicating cells of solid tissues. However, L-plastin is induced in many human solid tumor-derived cells. We used the anchored polymerase chain reaction technique to amplify and clone the missing 5' ends of plastin mRNAs. We found that both plastin isoforms contain a potential calcium binding site near the N terminus.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Calcium / metabolism*
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Calmodulin / genetics
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DNA / genetics
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Genetic Variation
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Humans
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Membrane Glycoproteins
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Microfilament Proteins
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Molecular Sequence Data
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Oligonucleotide Probes
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Phosphoproteins*
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Polymerase Chain Reaction
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Protein Binding
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Proteins / genetics*
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Proteins / metabolism
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RNA, Messenger / genetics
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Sequence Homology, Nucleic Acid
Substances
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Calmodulin
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Membrane Glycoproteins
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Microfilament Proteins
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Oligonucleotide Probes
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Phosphoproteins
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Proteins
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RNA, Messenger
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plastin
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DNA
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Calcium
Associated data
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GENBANK/M34426
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GENBANK/M34427