Using NanoDot dosimetry to study the RS 2000 X-ray biological irradiator

Int J Radiat Biol. 2013 Dec;89(12):1094-9. doi: 10.3109/09553002.2013.817703. Epub 2013 Jul 29.


Purpose: To use NanoDot dosimeters to study the RS 2000 X-ray Biological Irradiator dosimetry characteristics and perform in vivo dosimetry for cell or small animal experiments.

Methods and materials: We first calibrated the Landauer NanoDot(™) Reader by irradiating some NanoDot dosimeters with a set of known doses at specific positions defined by the irradiator. A group of five NanoDot dosimeters were placed at five specific positions where the dose rates were known and provided by the irradiator. Each group was irradiated for a set of times respectively. By correlating the readings of dosimeters with the given irradiated doses, we established the dose-reading relationship for the irradiator under the specific running condition. The established calibration curve was validated by exposing arbitrary known doses to a set of dosimeters, using the Landauer NanoDot(™) Reader to measure the doses, and then making the comparison between the two doses. To study the dose gradient of the X-ray inside the irradiated target (dose variation/cm), we placed dosimeters under different thicknesses of water-equivalent bolus and irradiated them, then measured the doses to determine the dose gradient.

Results: Using the method described above, we were able to calibrate the Landauer InLight NanoDot(™) Reader and use NanoDot dosimeters to measure the actual doses delivered to the targets for the cell/small animal experiments that use the RS 2000 X-ray Biological Irradiator.

Conclusions: NanoDots are ideal dosimeters to use for in vivo dosimetry for cell/small animal irradiation experiments. The dose decrease inside the animal tissue is about 20% per cm.

MeSH terms

  • Animals
  • Calibration
  • Equipment Design
  • Ions
  • Mice
  • Nanotechnology / methods
  • Radiation Dosage
  • Radiometry / instrumentation*
  • Radiometry / methods*
  • Scattering, Radiation
  • X-Rays*


  • Ions