TLR2 activation causes no morbidity or cardiovascular failure, despite excessive systemic nitric oxide production

Cardiovasc Res. 2013 Oct 1;100(1):28-35. doi: 10.1093/cvr/cvt168. Epub 2013 Jun 19.


Aims: Septic shock is the leading cause of death in intensive care units worldwide, resulting from a progressive systemic inflammatory reaction causing cardiovascular and organ failure. Nitric oxide (NO) is a potent vasodilator and inhibition of NO synthases (NOS) can increase blood pressure in septic shock. However, NOS inhibition does not improve outcome, on the contrary, and certain NO donors may even provide protection. In addition, NOS produce superoxide in case of substrate or cofactor deficiency or oxidation. We hypothesized that excessive systemic iNOS-derived NO production is insufficient to trigger cardiovascular failure and shock.

Methods and results: We found that the systemic injection with various synthetic Toll-like receptor-2 (TLR2), TLR3, or TLR9 agonists triggered systemic NO production identical to that of lipopolysaccharide (LPS) or tumour necrosis factor. In contrast to the latter, however, these agonists did not cause hypothermia or any other signs of discomfort or morbidity, and inflammatory cytokine production was low. TLR2 stimulation with the triacylated lipopeptide Pam3CSK4 not only caused identical NO levels in circulation, but also identical iNOS expression patterns as LPS. Nevertheless, Pam3CSK4 did not cause hypotension, bradycardia, reduced blood flow, or inadequate tissue perfusion in the kidney or the liver.

Conclusion: We demonstrate that excessive iNOS-derived NO in circulation is not necessarily linked to concomitant cardiovascular collapse, morbidity, or mortality. As such, our data indicate that the central role of iNOS-derived NO in inflammation-associated cardiovascular failure may be overestimated.

Keywords: Hypotension; LPS; NOS; Shock; TLR2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytokines / physiology
  • Female
  • Lipopolysaccharides / pharmacology
  • Mice
  • Mice, Inbred C57BL
  • Morbidity
  • Nitric Oxide / biosynthesis*
  • Nitric Oxide Synthase / physiology
  • Shock, Septic / etiology*
  • Systemic Inflammatory Response Syndrome / etiology
  • Toll-Like Receptor 2 / physiology*


  • Cytokines
  • Lipopolysaccharides
  • Tlr2 protein, mouse
  • Toll-Like Receptor 2
  • Nitric Oxide
  • Nitric Oxide Synthase