Propolis reduces oxidative stress in l-NAME-induced hypertension rats

Cell Biochem Funct. 2014 Mar;32(2):150-4. doi: 10.1002/cbf.2986. Epub 2013 Jun 21.


The inhibition in the synthesis or bioavailability of nitric oxide (NO) has an important role in progress of hypertension. The blocking of nitric oxide synthase activity may cause vasoconstriction with the formation of reactive oxygen species (ROS). Propolis is a resinous substance collected by honey bees from various plants. Propolis has biological and pharmacological properties. The aim of this study was to examine the effect of propolis on catalase (CAT) activity, malondialdehyde (MDA) and NO levels in the testis tissues of hypertensive rats by Nω-nitro-l-arginine methyl ester (l-NAME). Rats have received nitric oxide synthase inhibitor (l-NAME, 40 mg kg(-1) , intraperitoneally) for 15 days to produce hypertension and propolis (200 mg kg(-1) , by gavage) during the last 5 days. MDA level in l-NAME-treated group significantly increased compared with control group (P < 0.01). MDA level of l-NAME + propolis-treated rats significantly reduced (P < 0.01) compared with l-NAME-treated group. CAT activity and NO level significantly reduced (P < 0.01) in l-NAME group compared with control group. There were no statistically significant increases in the CAT activity and NO level of the l-NAME + propolis group compared with the l-NAME-treated group (P > 0.01). These results suggest that propolis changes CAT activity, NO and MDA levels in testis of l-NAME-treated animals, and so it may modulate the antioxidant system.

Keywords: Catalase; l-NAME; malondialdehyde; nitric oxide; oxidative stress; propolis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bees
  • Catalase / metabolism
  • Hypertension / metabolism
  • Hypertension / therapy*
  • Male
  • Malondialdehyde / metabolism
  • NG-Nitroarginine Methyl Ester / pharmacology*
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Nitric Oxide Synthase / metabolism
  • Oxidative Stress / drug effects*
  • Propolis / therapeutic use*
  • Rats
  • Rats, Wistar
  • Testis / metabolism


  • Nitric Oxide
  • Malondialdehyde
  • Propolis
  • Catalase
  • Nitric Oxide Synthase
  • NG-Nitroarginine Methyl Ester