Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition

J Cell Sci. 2013 Aug 15;126(Pt 16):3686-96. doi: 10.1242/jcs.126292. Epub 2013 Jun 20.


Notch signaling is an evolutionarily conserved pathway that plays a central role in numerous developmental and disease processes. The versatility of the Notch pathway relies on the activity of context-dependent regulators. These include rab11, sec15, arp3 and Drosophila EHBP1 (dEHBP1), which control Notch signaling and cell fate acquisition in asymmetrically dividing mechanosensory lineages by regulating the trafficking of the ligand Delta. Here, we show that dEHBP1 also controls the specification of R8 photoreceptors, as its loss results in the emergence of supernumerary R8 photoreceptors. Given the requirements for Notch signaling during lateral inhibition, we propose that dEHBP1 regulates distinct aspects of Notch signaling in different developmental contexts. We show that dEHBP1 regulates the exocytosis of Scabrous, a positive regulator of Notch signaling. In conclusion, dEHBP1 provides developmental versatility of intercellular signaling by regulating the trafficking of distinct Notch signaling components.

Keywords: Drosophila; EHBP1; Lateral inhibition; Notch; Photoreceptor development; Scabrous.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Animals
  • Cell Differentiation / physiology
  • Drosophila
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Insect Hormones / biosynthesis
  • Insect Hormones / genetics
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics*
  • Photoreceptor Cells, Invertebrate / metabolism
  • Protein Binding
  • Receptors, Notch / genetics
  • Receptors, Notch / metabolism*
  • Signal Transduction


  • Adaptor Proteins, Signal Transducing
  • Drosophila Proteins
  • EHD protein-binding protein 1, Drosophila
  • Insect Hormones
  • Membrane Proteins
  • N protein, Drosophila
  • Receptors, Notch