The practical analysis of food: the development of Sakalar quantification table of DNA (SQT-DNA)

Food Chem. 2013 Nov 15;141(2):718-22. doi: 10.1016/j.foodchem.2013.03.060. Epub 2013 Mar 25.

Abstract

Practical and highly sensitive Sakalar quantification table of DNA (SQT-DNA) has been developed for the detection% of species-specific DNA amount in food products. Cycle threshold (Ct) data were obtained from multiple curves of real-time qPCR. The statistical analysis was done to estimate the concentration of standard dilutions. Amplicon concentrations versus each Ct value were assessed by the predictions of targets at known concentrations. SQT-DNA was prepared by using the percentage versus each Ct values. The applicability of SQT-DNA to commercial foods was proved by using sausages containing varying ratios of beef, chicken, and soybean. The results showed that SQT-DNA can be used to directly quantify food DNA by a single PCR without the need to construct a standart curve in parallel with the samples every time the experiment is performed, and also quantification by SQT-DNA is as reliable as standard curve quantification for a wide range of DNA concentrations.

MeSH terms

  • Animals
  • Cattle / genetics
  • Chickens / genetics
  • DNA / genetics*
  • DNA / standards
  • Food Contamination / analysis*
  • Glycine max / genetics
  • Meat Products / analysis*
  • Real-Time Polymerase Chain Reaction / methods
  • Real-Time Polymerase Chain Reaction / standards*

Substances

  • DNA