The in vitro biosynthesis of dhurrin, the cyanogenic glycoside of Sorghum bicolor

J Biol Chem. 1975 Jun 25;250(12):4708-13.


A microsomal fraction from seedlings of Sorghum bicolor (Linn) Moench has been shown to catalyze the conversion of L-tyrosine to p-hydroxymandelonitrile via p-hydroxyphenylacetaldoxime. This transformation is consistent with the general pathway for cyanogenic glycoside biosynthesis proposed on the basis of in vivo experiments. When the microsomal fraction was combined with a protein fraction from the soluble portion of the cell and uridine diphosphate glucose, it was possible to demonstrate the synthesis of the cyanogenic glycoside dhurrin from L-tyrosine.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Chromatography, Paper
  • Glycosides / biosynthesis*
  • NADP
  • Nitriles / metabolism*
  • Oxidation-Reduction
  • Plants / metabolism*
  • Tyrosine / metabolism
  • Uridine Diphosphate Glucose


  • Glycosides
  • Nitriles
  • Tyrosine
  • NADP
  • Uridine Diphosphate Glucose