Circadian gene Clock contributes to cell proliferation and migration of glioma and is directly regulated by tumor-suppressive miR-124

FEBS Lett. 2013 Aug 2;587(15):2455-60. doi: 10.1016/j.febslet.2013.06.018. Epub 2013 Jun 19.

Abstract

Although the roles of circadian Clock genes and microRNAs in tumorigenesis have been profoundly studied, mechanisms of cross-talk between them in regulation of gliomagenesis are poorly understood. Here we show that the expression level of CLOCK is significantly increased in high-grade human glioma tissues and glioblastoma cell lines. In contrast miR-124 is attenuated in similar samples. Further studies show that Clock is a direct target of miR-124, and either restoration of miR-124 or silencing of CLOCK can reduce the activation of NF-κB. In conclusion, we suggest that as a target of glioma suppressor miR-124, CLOCK positively regulates glioma proliferation and migration by reinforcing NF-κB activity.

Keywords: CLOCK; Glioma; NF-κB; miR-124.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain Neoplasms / genetics
  • Brain Neoplasms / pathology*
  • CLOCK Proteins / physiology*
  • Cell Movement*
  • Cell Proliferation*
  • Circadian Rhythm / physiology*
  • Glioma / genetics
  • Glioma / pathology*
  • Humans
  • MicroRNAs / genetics*
  • Real-Time Polymerase Chain Reaction

Substances

  • MicroRNAs
  • CLOCK Proteins