Exosomes of BV-2 Cells Induced by Alpha-Synuclein: Important Mediator of Neurodegeneration in PD

Neurosci Lett. 2013 Aug 26;548:190-5. doi: 10.1016/j.neulet.2013.06.009. Epub 2013 Jun 17.

Abstract

Parkinson's disease (PD) is a progressive neurodegenerative disease. Alpha-synuclein aggregation, which can activate microglia to enhance its dopaminergic neurotoxicity, plays a central role in the progression of PD. However the mechanism is still unclear. To investigate how alpha-synuclein affects the neuron, exosomes were derived from alpha-synuclein treated mouse microglia cell line BV-2 cells by differential centrifugation and ultracentrifugation. We found that alpha-synuclein can induce an increase of exosomal secretion by microglia. These activated exosomes expressed a high level of MHC class II molecules and membrane TNF-α. In addition, the activated exosomes cause increased apoptosis. Exosomes secreted from activated microglias might be important mediator of alpha-synuclein-induced neurodegeneration in PD.

Keywords: APCs; Alpha-synuclein; CM; Exosome; FWB; LBs; Lewy bodies; Microglia; PD; Parkinson's disease; TACE; TNF-α converting enzyme; antigen-presenting cells; culture medium; flow cytometry wash buffer.

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Cell Line
  • Exosomes / drug effects
  • Exosomes / metabolism*
  • Mice
  • Microglia / drug effects
  • Microglia / metabolism*
  • Microglia / pathology*
  • Nerve Degeneration / metabolism*
  • Parkinson Disease / metabolism*
  • Parkinson Disease / pathology
  • alpha-Synuclein / metabolism*
  • alpha-Synuclein / pharmacology*

Substances

  • alpha-Synuclein