Multiplexed MS/MS for Improved Data-Independent Acquisition

Nat Methods. 2013 Aug;10(8):744-6. doi: 10.1038/nmeth.2528. Epub 2013 Jun 23.

Abstract

In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity fivefold.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Peptides / analysis*
  • Proteomics / methods*
  • Tandem Mass Spectrometry / methods*

Substances

  • Peptides