[Antimicrobial activity of stable silver nanoparticles of a certain size]

Prikl Biokhim Mikrobiol. Mar-Apr 2013;49(2):215-23. doi: 10.7868/s0555109913020128.
[Article in Russian]

Abstract

Conditions for obtaining stable silver nanoparticles smaller than 10 nm were developed using a binary stabilizer polyvinylpyrrolidone/sodium dodecylsulphate in optimal ratio. Optical spectra, morphology and dependence of size of the nanoparticles on the amount of reducing agent were studied. Colloidal solutions of nanosilver showed a high bactericidal activity against strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and fungicidal activity against Candida albicans. The mechanism of action of nanosized silver on microbial cell was examined by laser scanning confocal microscope using fluorescent label. First step of antimicrobial effect on microorganisms was membrane damage and penetration of silver nanoparticles into the cell. Prolonged stability of nanoparticles and their antimicrobial activity over the past two years were showed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacology*
  • Candida albicans / drug effects
  • Candida albicans / growth & development
  • Cell Membrane / drug effects*
  • Colloids
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Fluorescent Dyes
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / ultrastructure
  • Microbial Sensitivity Tests
  • Microbial Viability / drug effects
  • Microscopy, Confocal
  • Particle Size
  • Povidone / chemistry
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / growth & development
  • Silver / chemistry
  • Silver / pharmacology*
  • Sodium Dodecyl Sulfate / chemistry
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / growth & development

Substances

  • Anti-Bacterial Agents
  • Antifungal Agents
  • Colloids
  • Fluorescent Dyes
  • Sodium Dodecyl Sulfate
  • Silver
  • Povidone