Proposal for sequencing DNA using ligation of hexamers to generate sequential elongation primers (SPEL-6)

W Szybalski

doi:10.1016/0378-1119(90)90458-4

Proposal for sequencing DNA using ligation of hexamers to generate sequential elongation primers (SPEL-6)

Gene. 1990 May 31;90(1):177-8. doi: 10.1016/0378-1119(90)90458-4.

Author

W Szybalski¹

Affiliation

¹ McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.

PMID: 2379835
DOI: 10.1016/0378-1119(90)90458-4

Abstract

The efficiency of the directed-priming proposal of Studier [Proc. Natl. Acad. Sci. USA 86 (1989) 6917-1921] can be improved upon by employing a library of less than 4094 hexamers, allowing for the easy construction of all required primers using template-directed automated ligation. This 'top-down' sequencing procedure should be several times more efficient than the present 'bottom-up' procedures [see Watson, Science 248 (1990) 44-49].

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Sequence*
Human Genome Project
Oligonucleotide Probes
Temperature

Substances

Oligonucleotide Probes

Grants and funding

5-RO1-GM39715-02/GM/NIGMS NIH HHS/United States