Serum Vitamin B6, Folate, and Homocysteine Concentrations and Oxidative DNA Damage in Japanese Men and Women

Nutrition. 2013 Oct;29(10):1219-23. doi: 10.1016/j.nut.2013.03.014. Epub 2013 Jun 22.


Objective: Higher vitamin B status has been linked to a lower risk for cancer, but the underlying mechanism remains elusive. The aim of the present study was to examine the association of pyridoxal, folate, and homocysteine (Hcy) with urinary 8-hydroxydeoxyguanosine (8-OHdG), a marker of oxidative DNA damage.

Methods: The participants were 500 employees (293 men and 207 women), ages 21 to 66 y, of two municipal offices in Japan. Serum pyridoxal and Hcy concentrations were measured using high-performance liquid chromatography (HPLC) method, and serum folate concentrations were measured using chemiluminescent immunoassay. Urinary 8-OHdG concentrations were measured using HPLC method. Multiple regression was used to estimate means of 8-OHdG for each tertile of pyridoxal, folate, and Hcy with adjustment for potential confounders.

Results: In multivariate analysis, 8-OHdG concentration was inversely associated with pyridoxal concentration in men (P for trend = 0.045) but not in women. The association in men was confined to non-smokers (P for trend = 0.033) or those who consumed no or < 20 g/d of ethanol (P for trend = 0.048). 8-OHdG concentrations were not appreciably associated with folate and Hcy concentrations.

Conclusion: The results suggest that vitamin B6, but not folate and homocysteine, plays a role against oxidative DNA damage in Japanese men.

Keywords: Cross-sectional study; Oxidative DNA damage; Serum folate; Serum homocysteine; Serum vitamin B(6); Urinary 8-OHdG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Aged
  • Asian Continental Ancestry Group*
  • Biomarkers / blood
  • Biomarkers / urine
  • Chromatography, High Pressure Liquid
  • Cross-Sectional Studies
  • DNA Damage / drug effects*
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / urine
  • Female
  • Folic Acid / blood*
  • Homocysteine / blood*
  • Humans
  • Japan
  • Linear Models
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Oxidative Stress / drug effects*
  • Pyridoxal / blood
  • Vitamin B 6 / blood*
  • Young Adult


  • Biomarkers
  • Homocysteine
  • Pyridoxal
  • Vitamin B 6
  • 8-Hydroxy-2'-Deoxyguanosine
  • Folic Acid
  • Deoxyguanosine