Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid

PLoS One. 2013 Jun 21;8(6):e67592. doi: 10.1371/journal.pone.0067592. Print 2013.

Abstract

Noroviruses are major pathogens associated with acute gastroenteritis. They are diverse viruses, with at least six genogroups (GI-GVI) and multiple genotypes defined by differences in the major capsid protein, VP1. This diversity has challenged the development of broadly cross-reactive vaccines as well as efficient detection methods. Here, we report the characterization of a broadly cross-reactive monoclonal antibody (MAb) raised against the capsid protein of a GII.3 norovirus strain. The MAb reacted with VLPs and denatured VP1 protein from GI, GII, GIV and GV noroviruses, and mapped to a linear epitope located in the inner shell domain. An alignment of all available VP1 sequences showed that the putative epitope (residues 52-56) is highly conserved across the genus Norovirus. This broadly cross-reactive MAb thus constitutes a valuable reagent for the diagnosis and study of these diverse viruses.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / analysis
  • Antibodies, Monoclonal / immunology
  • Antibodies, Viral / analysis
  • Antibodies, Viral / immunology
  • Capsid / chemistry
  • Capsid / metabolism*
  • Capsid Proteins / genetics
  • Capsid Proteins / immunology
  • Capsid Proteins / metabolism
  • Chlorocebus aethiops
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / genetics
  • Epitopes / immunology
  • Epitopes / metabolism
  • Genotype
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Fluorescence
  • Mutagenesis
  • Norovirus / genetics
  • Norovirus / metabolism*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / immunology
  • Recombinant Proteins / isolation & purification
  • Sequence Alignment
  • Vero Cells
  • Viral Proteins / genetics
  • Viral Proteins / immunology
  • Viral Proteins / metabolism

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Capsid Proteins
  • Epitopes
  • Recombinant Proteins
  • Viral Proteins

Grant support

This research was partially supported by the Intramural Research Program of the NIH, NIAID. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding received for this study.