Sugar competition assays reveal high affinity receptors for Erythrina cristigalli lectin on feline monocytes

J Immunol Methods. 1990 Jul 20;131(1):15-24. doi: 10.1016/0022-1759(90)90227-m.

Abstract

An examination of fluorescein-labelled Erythrina cristigalli lectin (FITC-ECL) staining on feline mononuclear cells (MNC) using fluorescent microscopy and a novel sugar titration competition assay revealed that monocytes (MO) were stained brighter by FITC-ECL than were lymphocytes (LYM). When MNC were stained with FITC-ECL in the presence of 400 mM or greater D-galactose, analysis by flow cytometry revealed continued MO staining while LYM were negative. MO expressed a larger quantity of carbohydrate receptors (CHO-R) for ECL than did LYM. The CHO-R expressed on MO were mostly protease-insensitive and uncapped by sialic acid residues. All of the CHO-R on LYM were protease-sensitive and many were capped by sialic acid residues. A combined labelling of MNC for non-specific esterase staining, latex bead ingestion and FITC-ECL staining in the presence of 400 mM D-galactose confirmed that FITC-ECL specifically stains MO in the presence of high sugar competitor concentrations.

MeSH terms

  • Animals
  • Carbohydrate Metabolism
  • Cats
  • Erythrina
  • Flow Cytometry
  • Lectins / metabolism
  • Monocytes / metabolism*
  • Neuraminidase / pharmacology
  • Plant Lectins
  • Plants, Medicinal
  • Pronase / pharmacology
  • Receptors, Mitogen / metabolism*

Substances

  • Lectins
  • Plant Lectins
  • Receptors, Mitogen
  • erythrina lectin
  • Neuraminidase
  • Pronase