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. 2013 Sep;60:393-8.
doi: 10.1016/j.ijbiomac.2013.06.016. Epub 2013 Jun 24.

Anti-oxidant Activity of Holo- And Apo-C-Phycocyanin and Their Protective Effects on Human Erythrocytes

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Anti-oxidant Activity of Holo- And Apo-C-Phycocyanin and Their Protective Effects on Human Erythrocytes

Pornthip Pleonsil et al. Int J Biol Macromol. .

Abstract

This study was conducted to investigate the anti-oxidant activity of the recombinant apo-c-phycocyanin (c-PC) β-subunit compared to native c-PC purified from Spirulina sp. The gene encoding the β-subunit of c-PC was successfully cloned and expressed in Escherichia coli. The anti-oxidant capacities of recombinant apo-c-PC(β) and native c-PC were evaluated by measuring their Trolox equivalent antioxidant capacities and examining their protective effects on erythrocytes from normal and homozygous haemoglobin E individuals against peroxyl radicals and hydrogen peroxide. The results demonstrated that the anti-oxidant capacities are native c-PC≫Trolox>recombinant apo-c-PC(β). Both anti-oxidant proteins can potentially protect erythrocytes from oxidative damage. Expression of c-PC in bacteria reduces the cost and time for protein production, and the recombinant protein could be further developed to obtain a more efficient protein for therapeutic purposes.

Keywords: Anti-oxidant; Erythrocyte; Haemoglobin E; Phycocyanin; Recombinant protein.

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