Abstract
In this study, the potent skin-whitening effects of Octaphlorethol A (OPA) isolated from Ishige foliacea was investigated through inhibitory effect of melanin synthesis and tyrosinase activity in alpha-melanocyte stimulating hormone (α-MSH) induced B16F10 melanoma cells. OPA markedly inhibited melanin synthesis and tyrosinase activity in a concentration-dependent manner. We also found that OPA decreased microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 and -2 (TRP-1 and TRP-2) protein expressions. Moreover, OPA reduces p38 MAPK protein levels and activates extracellular signal-regulated kinase (ERK) and c-jun N-terminal kinases (JNKs) protein expressions in B16F10 cells. A specific ERK inhibitor PD98059 significantly blocks OPA-inhibited melanin synthesis and tyrosinase activity, whereas a p38MAP and JNK inhibitor had no effect. These findings provide evidence demonstrating that the anti-melanogenic effect of OPA is mediated through the activation of ERK signal pathway in B16F10 cells. These results indicate that OPA has the potential to be used as a melanogenesis inhibitor in the food and cosmetics industry.
Keywords:
Anti-melanogenesis; B16F10 melanoma cells; ERK; MITF; Octaphlorethol A (OPA).
Copyright © 2013 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line, Tumor
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Cell Survival / drug effects
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Down-Regulation / drug effects*
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Drug Discovery
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Intramolecular Oxidoreductases / antagonists & inhibitors
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Intramolecular Oxidoreductases / metabolism
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MAP Kinase Signaling System / drug effects
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Melanins / antagonists & inhibitors
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Melanins / biosynthesis*
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Melanocytes / drug effects*
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Melanocytes / enzymology
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Melanocytes / metabolism
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Membrane Glycoproteins / antagonists & inhibitors
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Membrane Glycoproteins / metabolism
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Mice
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Microphthalmia-Associated Transcription Factor / antagonists & inhibitors
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Microphthalmia-Associated Transcription Factor / metabolism
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Monophenol Monooxygenase / antagonists & inhibitors
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Monophenol Monooxygenase / metabolism
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Neoplasm Proteins / antagonists & inhibitors
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / metabolism
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Oxidoreductases / antagonists & inhibitors
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Oxidoreductases / metabolism
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Phaeophyceae / chemistry
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Phenols / adverse effects
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Phenols / antagonists & inhibitors
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Phenols / isolation & purification
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Phenols / pharmacology*
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Pigmentation / drug effects*
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Protein Kinase Inhibitors / pharmacology
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Skin Lightening Preparations / adverse effects
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Skin Lightening Preparations / chemistry
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Skin Lightening Preparations / isolation & purification
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Skin Lightening Preparations / pharmacology*
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alpha-MSH / antagonists & inhibitors*
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alpha-MSH / metabolism
Substances
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Melanins
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Membrane Glycoproteins
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Microphthalmia-Associated Transcription Factor
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Mitf protein, mouse
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Neoplasm Proteins
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Phenols
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Protein Kinase Inhibitors
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Skin Lightening Preparations
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octaphlorethol A
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alpha-MSH
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Oxidoreductases
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Tyrp1 protein, mouse
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Monophenol Monooxygenase
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Intramolecular Oxidoreductases
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dopachrome isomerase