Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR

J Microbiol Methods. 2013 Oct;95(1):32-8. doi: 10.1016/j.mimet.2013.06.003. Epub 2013 Jun 25.

Abstract

A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.

Keywords: Broiler; Campylobacter; PMA; qPCR.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Azides / metabolism*
  • Bacterial Load / methods*
  • Campylobacter coli / isolation & purification*
  • Campylobacter coli / physiology
  • Campylobacter jejuni / isolation & purification*
  • Campylobacter jejuni / physiology
  • Campylobacter lari / isolation & purification*
  • Campylobacter lari / physiology
  • Chickens / microbiology*
  • Microbial Viability
  • Propidium / analogs & derivatives*
  • Propidium / metabolism
  • Real-Time Polymerase Chain Reaction / methods*

Substances

  • Azides
  • propidium monoazide
  • Propidium