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, 18 (7), 7570-83

Hepatotoxicity Evaluation of Aqueous Extract From Scutia Buxifolia

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Hepatotoxicity Evaluation of Aqueous Extract From Scutia Buxifolia

Robson Borba de Freitas et al. Molecules.

Abstract

Nowadays there is an increase in the number of people taking herbals worldwide. Scutia buxifolia is used for the treatment of hypertension, but little is known about its action on liver. Thirty-two Wistar rats were divided into four groups: control and groups treated during 30 days with 100, 200 and 400 mg of lyophilized aqueous extract of S. buxifolia (SBSB)/kg of body weight. This study was planned to explore hepatotoxic effect of SBSB, which was assessed by serum transaminases (ALT and AST). Thiobarbituric acid reactive substances (TBARS) levels were determined in liver, along with thiols content (NPSH), catalase (CAT) activity and, superoxide dismutase (SOD) enzymes. Histopathological studies of liver tissue were performed. Flavonoids and phenolics were quantified in SBSB by high performance liquid chromatography with diode array detection (HPLC/DAD). We did not observe alterations on redox status (TBARS, NPSH, CAT and, SOD) in the control and experimental groups. An increase on AST activity was only observed at 200 mg of SBSB, whereas ALT score was not affected by SBSB. Moreover, no morphological alterations were observed on the hepatocytes, matching the analysed biochemical parameters. This way, we conclude that SBSB was not toxic.

Figures

Figure 1
Figure 1
Representative high performance liquid chromatography profile of the S. buxifolia lyophilized extract. Gallic acid (1), chlorogenic acid (2), caffeic acid (3), rutin (4), quercetin (5) and kaempferol (6).
Figure 2
Figure 2
Asparate aminotransferase (AST) activity (A); alanine aminotransferase (ALT) activity (B).
Figure 3
Figure 3
Thiobarbituric acid reactive substances (TBARS) levels expressed in nmol MDA/mg protein. No significant difference was noted between control group and 100, 200, 400 mg of SBSB/kg body weight treated groups (p < 0.05). Data are expressed in mean ± SD.
Figure 4
Figure 4
Tissue sulfhydryl groups content (NPSH). No significant difference was noted between control group and 100, 200, 400 mg of SBSB/kg body weight treated groups (p < 0.05). Data are expressed in mean ± SD.
Figure 5
Figure 5
Catalase (CAT) activity. No significant difference was noted between control group and 100, 200, 400 mg of SBSB/kg body weight treated groups (p < 0.05). Data are expressed in mean ± SD.
Figure 6
Figure 6
Superoxide dismutase (SOD) activity. No significant difference was noted between control group and 100, 200, 400 mg of SBSB/kg body weight treated groups (p < 0.05). Data are expressed in mean ± SD.
Figure 7
Figure 7
Light micrograph (H & E staining) of liver. (A) control group (400×); (B) 100 mg of SBSB/weight treated group (400×); (C) 200 mg of SBSB/weight treated group (400×); (D) 400 mg of SBSB/weight treated group (400×). Figures show the centrilobular vein. There is presence of red blood cells on (C) and (D).

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