Exosomes from human adipose-derived mesenchymal stem cells promote migration through Wnt signaling pathway in a breast cancer cell model

Mol Cell Biochem. 2013 Nov;383(1-2):13-20. doi: 10.1007/s11010-013-1746-z. Epub 2013 Jun 29.

Abstract

Human mesenchymal stem cell (MSC)-conditioned medium (CM) was previously reported to affect the biology of tumor cells; however, the precise mechanisms remain unclear. Here, we show that MSCs secreted 40-100 nm particles, which have the typical characteristics of exosomes, and these MSC-derived exosomes promoted migration of the breast cancer cell line MCF7. Global gene expression profiling revealed that several cancer-related signaling pathways were upregulated after exosome treatment in MCF7, and the Wnt signaling pathway was further confirmed to be activated. Our findings demonstrated a new mechanism through which MSC-CM may contribute to tumor cell migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology*
  • Breast Neoplasms / genetics
  • Breast Neoplasms / pathology*
  • Cell Movement*
  • Cell Proliferation
  • Exosomes / metabolism*
  • Exosomes / ultrastructure
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MCF-7 Cells
  • Mesenchymal Stem Cells / metabolism*
  • Models, Biological*
  • Wnt Signaling Pathway* / genetics