Molecular characteristics and evolutionary analysis of field Marek's disease virus prevalent in vaccinated chicken flocks in recent years in China

Virus Genes. 2013 Oct;47(2):282-91. doi: 10.1007/s11262-013-0942-y. Epub 2013 Jun 29.

Abstract

Marek's disease is a highly contagious, oncogenic, and immunosuppressive avian viral disease. Surveillance of newly registered Marek's disease virus (MDV) isolates is meaningful for revealing the potential factors involved in increased virulence. Presently, we have focused on the molecular characteristics of all available MDVs from China, including 17 new Henan isolates. Based on Meq, gE, and gI genes, we found that most Chinese isolates contain conserved amino acid point mutations in Meq, such as E(77), A(115), A(139), R(176), and A(217), compared to USA virulent MDVs. However, the 59-aa or 60-aa insertions are only found in a few mild MDVs rather than virulent MDVs in China. Further phylogenetic analysis has demonstrated that a different genotype of MDV has been prevalent in China, and for virulent MDVs, their recent evolution has possibly been geographically restricted. Our study has provided more detailed information regarding the field MDVs circulating in China.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • China
  • Cluster Analysis
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Evolution, Molecular
  • Mardivirus / classification
  • Mardivirus / genetics*
  • Mardivirus / isolation & purification*
  • Marek Disease / virology*
  • Molecular Sequence Data
  • Mutation, Missense
  • Phylogeny
  • Point Mutation
  • Sequence Analysis, DNA
  • Viral Proteins / genetics

Substances

  • DNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/HF546084
  • GENBANK/HF546085
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