Effects of one-time and two-time intra-articular injection of hyaluronic acid sodium salt after joint surgery in dogs

J Vet Sci. 2013;14(2):215-22. doi: 10.4142/jvs.2013.14.2.215. Epub 2013 Jun 21.


Thirty-one dogs with patellar luxation (grades 2 and 3) were categorized into three groups. Group 1 (G.1; n = 12) had sodium hyaluronate (SHA) intra-articularly injected into the stifle joint that received surgery. Group 2 (G.2; n = 10) received SHA twice: first after surgery and then 1 week later. Group 3 (G.3; n = 9) served as a control, without injection. Blood was collected before injection and then once a week for 4 weeks after injection for evaluation of chondroitin sulfate (CS-WF6) and hyaluronan (HA). The results revealed significantly (p < 0.05) improved clinical scores by the end of week 4 in G.1 and G.2 relative to G.3; however, there was no significant difference between G.1 and G.2. There was a significant decrease (p < 0.05) in serum CS-WF6 levels beginning at week 2 in G.1 and G.2. At weeks 3 and 4, serum HA in G.1 and G.2 differed from that in G.3 (p < 0.05). No significant difference (p > 0.05) was observed in serum biomarkers between G.1 and G.2. In conclusion, intra-articular injection with SHA after joint surgery may improve homeostasis of the joint, retarding the process of OA.

Keywords: biomarker; dog; joint surgery; osteoarthritis; sodium hyaluronate.

MeSH terms

  • Animals
  • Blood Chemical Analysis / veterinary
  • Chondroitin Sulfates / metabolism
  • Dogs*
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Female
  • Hyaluronic Acid / administration & dosage*
  • Hyaluronic Acid / metabolism
  • Injections, Intra-Articular / veterinary
  • Male
  • Osteoarthritis, Knee / drug therapy
  • Osteoarthritis, Knee / prevention & control
  • Osteoarthritis, Knee / veterinary*
  • Stifle / surgery*
  • Viscosupplements / administration & dosage*


  • Viscosupplements
  • Hyaluronic Acid
  • Chondroitin Sulfates