Metabolic Potential of the Organic-Solvent Tolerant Pseudomonas Putida DOT-T1E Deduced From Its Annotated Genome

Microb Biotechnol. 2013 Sep;6(5):598-611. doi: 10.1111/1751-7915.12061. Epub 2013 Jul 1.


Pseudomonas putida DOT-T1E is an organic solvent tolerant strain capable of degrading aromatic hydrocarbons. Here we report the DOT-T1E genomic sequence (6,394,153 bp) and its metabolic atlas based on the classification of enzyme activities. The genome encodes for at least 1751 enzymatic reactions that account for the known pattern of C, N, P and S utilization by this strain. Based on the potential of this strain to thrive in the presence of organic solvents and the subclasses of enzymes encoded in the genome, its metabolic map can be drawn and a number of potential biotransformation reactions can be deduced. This information may prove useful for adapting desired reactions to create value-added products. This bioengineering potential may be realized via direct transformation of substrates, or may require genetic engineering to block an existing pathway, or to re-organize operons and genes, as well as possibly requiring the recruitment of enzymes from other sources to achieve the desired transformation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotransformation
  • DNA, Bacterial / chemistry*
  • DNA, Bacterial / genetics*
  • Genome, Bacterial*
  • Metabolic Networks and Pathways / genetics*
  • Molecular Sequence Data
  • Pseudomonas putida / genetics*
  • Pseudomonas putida / metabolism*
  • Sequence Analysis, DNA*


  • DNA, Bacterial