Engineered Nanostructured β-sheet Peptides Protect Membrane Proteins

Nat Methods. 2013 Aug;10(8):759-61. doi: 10.1038/nmeth.2533. Epub 2013 Jun 30.

Abstract

We designed β-strand peptides that stabilize integral membrane proteins (IMPs). β-strand peptides self-assemble in solution as filaments and become restructured upon association with IMPs; resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. β-strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding cassette (ABC) transporter MsbA.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • ATP-Binding Cassette Transporters / chemistry
  • Bacterial Proteins / chemistry
  • Circular Dichroism
  • Membrane Proteins / chemical synthesis
  • Membrane Proteins / chemistry*
  • Microscopy, Electron, Transmission
  • Nanostructures / chemistry*
  • Peptides / chemical synthesis*
  • Peptides / chemistry*
  • Protein Structure, Secondary
  • Spectroscopy, Fourier Transform Infrared

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • Membrane Proteins
  • MsbA protein, Bacteria
  • Peptides