Heritable genome editing in C. elegans via a CRISPR-Cas9 system

Nat Methods. 2013 Aug;10(8):741-3. doi: 10.1038/nmeth.2532. Epub 2013 Jun 30.

Abstract

We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Genetic Engineering / methods
  • Genome*
  • Inverted Repeat Sequences*
  • Promoter Regions, Genetic
  • RNA, Guide / genetics*
  • RNA, Small Nuclear / genetics*
  • Zebrafish Proteins / genetics

Substances

  • RNA, Guide
  • RNA, Small Nuclear
  • Zebrafish Proteins
  • unc119a protein, zebrafish