Isolation and microsequence analysis of a novel form of neuromedin U from guinea pig small intestine

R Murphy; C A Turner; J B Furness; L Parker; A Giraud

doi:10.1016/0196-9781(90)90066-e

Isolation and microsequence analysis of a novel form of neuromedin U from guinea pig small intestine

Peptides. 1990 May-Jun;11(3):613-7. doi: 10.1016/0196-9781(90)90066-e.

Authors

R Murphy¹, C A Turner, J B Furness, L Parker, A Giraud

Affiliation

¹ Department of Anatomy and Histology, School of Medicine, Flinders University, Bedford Park, S.A., Australia.

PMID: 2381877
DOI: 10.1016/0196-9781(90)90066-e

Abstract

A multidimensional chromatographic regimen has been used to isolate and purify a peptide showing immunoreactivity for neuromedin U from guinea pig small intestine. Microsequence Edman N-terminal analysis and C-terminal analysis by enzymatic digestion showed this peptide to be a nonapeptide with the following sequence: H-Gly-Tyr-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The C-terminal octapeptide of this sequence is the same as porcine NMU-8, and the C-terminal heptapeptide is identical to rat NMU(17-23).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Chromatography, Gel
Guinea Pigs
Intestine, Small / analysis*
Molecular Sequence Data
Neuropeptides* / isolation & purification
Radioimmunoassay
Rats
Sequence Homology, Nucleic Acid
Swine

Substances

Neuropeptides
neuromedin U