Isolation and microsequence analysis of a novel form of neuromedin U from guinea pig small intestine

Peptides. 1990 May-Jun;11(3):613-7. doi: 10.1016/0196-9781(90)90066-e.


A multidimensional chromatographic regimen has been used to isolate and purify a peptide showing immunoreactivity for neuromedin U from guinea pig small intestine. Microsequence Edman N-terminal analysis and C-terminal analysis by enzymatic digestion showed this peptide to be a nonapeptide with the following sequence: H-Gly-Tyr-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The C-terminal octapeptide of this sequence is the same as porcine NMU-8, and the C-terminal heptapeptide is identical to rat NMU(17-23).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatography, Gel
  • Guinea Pigs
  • Intestine, Small / analysis*
  • Molecular Sequence Data
  • Neuropeptides* / isolation & purification
  • Radioimmunoassay
  • Rats
  • Sequence Homology, Nucleic Acid
  • Swine


  • Neuropeptides
  • neuromedin U