Novel polyomaviruses of nonhuman primates: genetic and serological predictors for the existence of multiple unknown polyomaviruses within the human population

PLoS Pathog. 2013;9(6):e1003429. doi: 10.1371/journal.ppat.1003429. Epub 2013 Jun 20.

Abstract

Polyomaviruses are a family of small non-enveloped DNA viruses that encode oncogenes and have been associated, to greater or lesser extent, with human disease and cancer. Currently, twelve polyomaviruses are known to circulate within the human population. To further examine the diversity of human polyomaviruses, we have utilized a combinatorial approach comprised of initial degenerate primer-based PCR identification and phylogenetic analysis of nonhuman primate (NHP) polyomavirus species, followed by polyomavirus-specific serological analysis of human sera. Using this approach we identified twenty novel NHP polyomaviruses: nine in great apes (six in chimpanzees, two in gorillas and one in orangutan), five in Old World monkeys and six in New World monkeys. Phylogenetic analysis indicated that only four of the nine chimpanzee polyomaviruses (six novel and three previously identified) had known close human counterparts. To determine whether the remaining chimpanzee polyomaviruses had potential human counterparts, the major viral capsid proteins (VP1) of four chimpanzee polyomaviruses were expressed in E. coli for use as antigens in enzyme-linked immunoassay (ELISA). Human serum/plasma samples from both Côte d'Ivoire and Germany showed frequent seropositivity for the four viruses. Antibody pre-adsorption-based ELISA excluded the possibility that reactivities resulted from binding to known human polyomaviruses. Together, these results support the existence of additional polyomaviruses circulating within the human population that are genetically and serologically related to existing chimpanzee polyomaviruses.

Publication types

  • Clinical Trial
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Capsid Proteins / blood
  • Capsid Proteins / genetics*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Male
  • Monkey Diseases / blood
  • Monkey Diseases / genetics*
  • Phylogeny*
  • Platyrrhini / blood
  • Platyrrhini / virology*
  • Polyomavirus / genetics*
  • Polyomavirus / metabolism
  • Polyomavirus Infections / blood
  • Polyomavirus Infections / genetics*

Substances

  • Antibodies, Viral
  • Capsid Proteins
  • VP1 protein, polyomavirus

Grant support

This work was supported by the “Deutsche Forschungsgemeinschaft” grant number LE1813/4-1. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.