Abstract
Isorosthin A (1), the first 20-nor-enmein-type diterpenoid, and 15 new ent-kauranoids, isorosthins B-P (2-16), along with 22 known analogues were isolated from the aerial parts of Isodon rosthornii. The structures of 1-16 were elucidated by means of spectroscopic analysis. The relative configuration of 2 and the absolute configuration of 3 were determined by single-crystal X-ray diffraction. Cytotoxicity evaluation against five human tumor lines showed inhibitory effects by several of the compounds tested. Furthermore, 12 of the isolates exhibited inhibitory activity against nitric oxide production in LPS-activated RAW264.7 macrophages.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology*
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Crystallography, X-Ray
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Diterpenes, Kaurane / chemistry
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Diterpenes, Kaurane / isolation & purification*
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Diterpenes, Kaurane / pharmacology*
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Drug Screening Assays, Antitumor
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology*
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Humans
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Isodon / chemistry*
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Mice
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Molecular Conformation
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Molecular Structure
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Nitric Oxide / analysis
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Nuclear Magnetic Resonance, Biomolecular
Substances
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Antineoplastic Agents, Phytogenic
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Diterpenes, Kaurane
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Drugs, Chinese Herbal
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Lipopolysaccharides
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isorosthin A
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Nitric Oxide