Quantification of microRNA expression with next-generation sequencing

Curr Protoc Mol Biol. 2013 Jul;Chapter 4:Unit 4.17. doi: 10.1002/0471142727.mb0417s103.


Rapid advancement of next-generation sequencing technologies has made it possible to study expression profiles of microRNAs (miRNAs) comprehensively and efficiently. Multiplexing miRNA libraries by barcoding can significantly reduce sequencing cost per sample without compromising library quality. This unit provides a step-by-step protocol for isolating miRNAs and constructing multiplexed miRNA libraries. Also described is a custom computational pipeline for analyzing the multiplexed miRNA library sequencing reads generated by Illumina-based technology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computational Biology / methods
  • Gene Expression Profiling / methods*
  • Gene Library
  • High-Throughput Nucleotide Sequencing / methods*
  • MicroRNAs / biosynthesis*
  • MicroRNAs / genetics
  • MicroRNAs / isolation & purification


  • MicroRNAs