[Mitokorrektine stimulates angiogenesis in vitro]

Fiziol Zh (1994). 2013;59(2):52-8.
[Article in Ukrainian]

Abstract

The effect of mitokorrectine (complex native oligopeptides isolated from neonatal pig brain) on endothelial cells in culture was investigated. It was revealed that the drug concentration-dependently induces angiogenesis in vitro. Mitogen effect of Mitokorrectine was shown by MTT-test and routine cell count in concentration diapason (0.1-1 mg/ml) which means an increased number of cells by 25 +/- 5% and cell subpopulation of proliferative pool (G2/M+S) 1,8 times in concentration diapason mitokorrectine (0.01-0.05 mg/ml) in comparison with control. In 3-D culture and in stationary phase we detected induction of differentiation of endothelial cells, a decrease the level of NO production and enhancement of glucose metabolism and stimulation of formation of capillary-like tubes.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Animals, Newborn
  • Brain Chemistry
  • Cell Cycle / drug effects
  • Cell Differentiation / drug effects
  • Cell Line
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects*
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Endothelial Cells / cytology
  • Endothelial Cells / drug effects*
  • Endothelial Cells / metabolism
  • Flow Cytometry
  • Glucose / metabolism
  • Liver / chemistry
  • Mitochondria / chemistry
  • Neovascularization, Physiologic / drug effects*
  • Nitric Oxide / metabolism
  • Oligopeptides / pharmacology*
  • Pancreas / chemistry
  • Swine

Substances

  • Oligopeptides
  • Nitric Oxide
  • Glucose