Isolation and structure assignment of an antimicrobial principle from the volatile oil of Nigella sativa L. seeds

Pharmazie. 1975 Feb;30(2):109-11.


Refrigeration of the volatile oil of Nigella sativa L. seeds eventuates in a crystalline substance. The chemical structure of the compound was drawn from its chemical behaviour, as well as from its UV, IR, PMR and mass spectral data. The compound was found to be thymohydroquinone: confirmation of the structure was established via the preparation of its corresponding diacetate ester. The compound was found to have high antimicrobial effect against gram positive microorganisms

MeSH terms

  • Bacillus subtilis / drug effects
  • Candida albicans / drug effects
  • Escherichia coli / drug effects
  • Microbial Sensitivity Tests
  • Micrococcus / drug effects
  • Oils, Volatile / analysis*
  • Plants, Medicinal / analysis*
  • Pseudomonas aeruginosa / drug effects
  • Salmonella paratyphi A / drug effects
  • Sarcina / drug effects
  • Seeds / analysis
  • Staphylococcus / drug effects
  • Thymol / analogs & derivatives*
  • Thymol / isolation & purification
  • Thymol / pharmacology


  • Oils, Volatile
  • thymohydroquinone
  • Thymol