This study evaluated the influence of bleaching gel pH and the effect of remineralizing gels after bleaching in different time intervals. Sixty bovine incisors were divided into 2 groups (n = 30). Group 1 was bleached with a 35% hydrogen peroxide (HP) acid gel and Group 2 was bleached with a 35% HP neutral gel. Each group was then divided into 3 subgroups (n = 10) according to the post-bleaching treatment used: storage in artificial saliva, application of a fluoride gel, or application of a gel consisting of fluoride, potassium nitrate, and nanostructured calcium phosphate. Specimens were stored in artificial saliva, and enamel microhardness was evaluated at 24 hours and 15 days postbleaching. Vickers microhardness data were analyzed by means of 2-way ANOVA, with repeated measurements and Bonferroni's post-hoc test. Twenty-four hours after bleaching, no significant differences were found between the bleaching gels. At 15 days postbleaching, Group 2 samples demonstrated a significant reduction in microhardness. No significant differences were found between the remineralizing gels, though all of the postbleaching treatments after the use of 35% neutral gel were able to re-establish baseline microhardness. It was concluded that neutral bleaching gel significantly reduced enamel microhardness 15 days after bleaching and that the use of remineralizing gels did not significantly enhance the microhardness of bleached enamel. However, in clinical situations, the acquired enamel pellicle protects tooth surfaces, and postbleaching, decalcified enamel would undergo recalcification. This study indicates that it is important to consider the bleaching agent's pH and composition when treating patients with reduced salivary secretion.