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. 2013 Jul 3;9(4):20130432.
doi: 10.1098/rsbl.2013.0432. Print 2013 Aug 23.

Deleterious Consequences of Antioxidant Supplementation on Lifespan in a Wild-Derived Mammal

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Free PMC article

Deleterious Consequences of Antioxidant Supplementation on Lifespan in a Wild-Derived Mammal

Colin Selman et al. Biol Lett. .
Free PMC article

Abstract

While oxidative damage owing to reactive oxygen species (ROS) often increases with advancing age and is associated with many age-related diseases, its causative role in ageing is controversial. In particular, studies that have attempted to modulate ROS-induced damage, either upwards or downwards, using antioxidant or genetic approaches, generally do not show a predictable effect on lifespan. Here, we investigated whether dietary supplementation with either vitamin E (α-tocopherol) or vitamin C (ascorbic acid) affected oxidative damage and lifespan in short-tailed field voles, Microtus agrestis. We predicted that antioxidant supplementation would reduce ROS-induced oxidative damage and increase lifespan relative to unsupplemented controls. Antioxidant supplementation for nine months reduced hepatic lipid peroxidation, but DNA oxidative damage to hepatocytes and lymphocytes was unaffected. Surprisingly, antioxidant supplementation significantly shortened lifespan in voles maintained under both cold (7 ± 2°C) and warm (22 ± 2°C) conditions. These data further question the predictions of free-radical theory of ageing and critically, given our previous research in mice, indicate that similar levels of antioxidants can induce widely different interspecific effects on lifespan.

Keywords: ageing; longevity; oxidative damage; vitamin C; vitamin E; vole.

Figures

Figure 1.
Figure 1.
Kaplan–Meier survival curves of voles maintained in the cold (a) 7 ± 2°C or warm (b) 22 ± 2°C and given access to either a control diet, a vitamin E-supplemented diet or a vitamin C-supplemented diet from two months of age. (a) Solid blue line denotes control, stippled green line denotes vitamin E and broken orange line denotes vitamin C groups. (b) Solid red line denotes control, stippled green line denotes vitamin E and broken orange line denotes vitamin C groups.
Figure 2.
Figure 2.
Mean (±s.e.m.) daily food intake ((a) g d−1), body mass ((b) g) and hepatic lipid peroxidation ((c) ηmol mg protein−1) levels at 11 months of age in vitamin E- and vitamin C-supplemented voles maintained in the cold and warm relative to their respective unsupplemented controls. Asterisk (*) denotes significant difference to appropriate control where p < 0.05.

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