Host factor SAMHD1 restricts DNA viruses in non-dividing myeloid cells

PLoS Pathog. 2013;9(6):e1003481. doi: 10.1371/journal.ppat.1003481. Epub 2013 Jun 27.


SAMHD1 is a newly identified anti-HIV host factor that has a dNTP triphosphohydrolase activity and depletes intracellular dNTP pools in non-dividing myeloid cells. Since DNA viruses utilize cellular dNTPs, we investigated whether SAMHD1 limits the replication of DNA viruses in non-dividing myeloid target cells. Indeed, two double stranded DNA viruses, vaccinia and herpes simplex virus type 1, are subject to SAMHD1 restriction in non-dividing target cells in a dNTP dependent manner. Using a thymidine kinase deficient strain of vaccinia virus, we demonstrate a greater restriction of viral replication in non-dividing cells expressing SAMHD1. Therefore, this study suggests that SAMHD1 is a potential innate anti-viral player that suppresses the replication of a wide range of DNA viruses, as well as retroviruses, which infect non-dividing myeloid cells.

Publication types

  • Clinical Trial
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Female
  • Herpesvirus 1, Human / physiology*
  • Humans
  • Male
  • Monomeric GTP-Binding Proteins / genetics
  • Monomeric GTP-Binding Proteins / metabolism*
  • Myeloid Cells / metabolism*
  • Myeloid Cells / pathology
  • Myeloid Cells / virology*
  • SAM Domain and HD Domain-Containing Protein 1
  • Vaccinia virus / physiology*
  • Virus Replication / physiology*


  • SAM Domain and HD Domain-Containing Protein 1
  • SAMHD1 protein, human
  • Monomeric GTP-Binding Proteins