L-mimosine and dimethyloxaloylglycine decrease plasminogen activation in periodontal fibroblasts

J Periodontol. 2014 Apr;85(4):627-35. doi: 10.1902/jop.2013.120703. Epub 2013 Jul 4.


Background: The use of prolyl hydroxylase inhibitors such as l-mimosine (L-MIM) and dimethyloxaloylglycine (DMOG) to improve angiogenesis is a new approach for periodontal regeneration. In addition to exhibiting pro-angiogenic effects, prolyl hydroxylase inhibitors can modulate the plasminogen activator system in cells from non-oral tissues. This study assesses the effect of prolyl hydroxylase inhibitors on plasminogen activation by fibroblasts from the periodontium.

Methods: Gingival and periodontal ligament fibroblasts were incubated with L-MIM and DMOG. To investigate whether prolyl hydroxylase inhibitors modulate the net plasminogen activation, kinetic assays were performed with and without interleukin (IL)-1. Moreover, plasminogen activators and the respective inhibitors were analyzed by casein zymography, immune assays, and quantitative polymerase chain reaction.

Results: The kinetic assay showed that L-MIM and DMOG reduced plasminogen activation under basal and IL-1-stimulated conditions. Casein zymography revealed that the effect of L-MIM involves a decrease in urokinase-type plasminogen activator activity. In agreement with these findings, reduced levels of urokinase-type plasminogen activator and elevated levels of plasminogen activator inhibitor 1 were observed.

Conclusion: L-MIM and DMOG can reduce plasminogen activation by fibroblasts from the gingiva and the periodontal ligament under basal conditions and in the presence of an inflammatory cytokine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Amino Acids, Dicarboxylic / pharmacology*
  • Cell Culture Techniques
  • Cells, Cultured
  • Female
  • Fibroblasts / drug effects*
  • Gingiva / cytology
  • Gingiva / drug effects
  • Humans
  • Interleukin-1 / pharmacology
  • Male
  • Mimosine / pharmacology*
  • Periodontal Ligament / cytology*
  • Periodontal Ligament / drug effects
  • Plasminogen Activator Inhibitor 1 / analysis
  • Plasminogen Activators / pharmacology
  • Plasminogen Inactivators / pharmacology*
  • Prolyl-Hydroxylase Inhibitors / pharmacology
  • Urokinase-Type Plasminogen Activator / antagonists & inhibitors


  • Amino Acids, Dicarboxylic
  • Interleukin-1
  • Plasminogen Activator Inhibitor 1
  • Plasminogen Inactivators
  • Prolyl-Hydroxylase Inhibitors
  • SERPINE1 protein, human
  • Mimosine
  • Plasminogen Activators
  • Urokinase-Type Plasminogen Activator
  • oxalylglycine