Effects of caveolin-1 and P-ERK1/2 on Ang II-induced glomerular mesangial cell proliferation

Ren Fail. 2013 Aug;35(7):971-7. doi: 10.3109/0886022X.2013.808956. Epub 2013 Jul 5.


This study explored the effects of caveolin-1, p-ERK1/2 and transient receptor potential channel 6 (TRPC6) on angiotensin II (Ang II)-induced glomerular mesangial cell (GMC) proliferation, and investigated the role of Ang II on GMC proliferation. GMC cultures were divided into Control, Ang II (Ang II 10(-7 )mol/L), PD98059 (Ang II 10(-7 )mol/L + PD98059 5 × 10(-5 )mol/L) and MβCD groups (Ang II 10(-7 )mol/L + MβCD 10(-2 )mol/L). GMCs proliferation was measured by the methyl thiazolil tetracolium and trypan blue assays. The distribution of caveolin-1, p-ERK1/2 and TRPC6 was monitored by immunocytochemistry. Real time polymerase chain reaction (PCR) was used to assess mRNA expression of caveolin-1 and TRPC6. Western blot analysis was used to assess protein expression of caveolin-1, p-ERK1/2 and TRPC6. The results showed that Ang II promoted GMC proliferation. PD98059 and MβCD blocked Ang II-induced GMC proliferation, by 31.06% and 48.96%, respectively. In comparison with the control group, the expression of p-ERK1/2 and TRPC6 was significantly higher and caveolin-1 expression was significantly lower in the Ang II group. PD98059 markedly decreased p-ERK1/2 and TRPC6 expression and increased caveolin-1 expression. MβCD decreased the expression of p-ERK1/2 and TRPC6, but had no significant effect on caveolin-1 protein expression. These findings suggested that the intact caveolae structure was associated with Ang II-induced GMC proliferation, ERK1/2 activation and TRPC6 expression. And p-ERK1/2 acted as an upstream signal molecule for TRPC6. Moreover, p-ERK1/2 and caveolin-1 appeared to be inhibited reciprocally, thus regulated GMC proliferation by regulating TRPC6 expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II / physiology*
  • Animals
  • Caveolin 1 / metabolism*
  • Cell Proliferation
  • Cells, Cultured
  • Extracellular Signal-Regulated MAP Kinases / metabolism*
  • Gene Expression Regulation
  • Immunohistochemistry
  • Mesangial Cells / physiology*
  • Rats
  • Signal Transduction
  • TRPC Cation Channels / metabolism


  • Caveolin 1
  • TRPC Cation Channels
  • Trpc6 protein, rat
  • Angiotensin II
  • Extracellular Signal-Regulated MAP Kinases