An immunoglobulin G complexed form of thyroid-stimulating hormone (macro thyroid-stimulating hormone) is a cause of elevated serum thyroid-stimulating hormone concentration

Ann Clin Biochem. 2013 Sep;50(Pt 5):416-20. doi: 10.1177/0004563213476271. Epub 2013 Jul 4.


Background: Macrocomplexes can be the cause of elevated serum hormone concentrations and may cause diagnostic confusion. This is well recognized for prolactin and commonly screened for using polyethylene glycol (PEG) precipitation. The phenomenon and a suitable screening method is less familiar with respect to thyroid-stimulating hormone (TSH).

Method: Samples sent to the laboratory for routine analysis of thyroid function and found to have a TSH >10 mU/L were evaluated to determine the prevalence of macro-TSH in the Roche Elecsys assay, using PEG precipitation with confirmation by gel filtration chromatography.

Results: Of 495 samples tested, 3 (0.6%) were found to have macro-TSH. From the distribution of recoveries, a cut-off <25% was determined for identifying samples requiring further investigation for the presence of macro-TSH.

Conclusion: The prevalence of elevated TSH due to macro-TSH was found to be 0.6%. Laboratories should be aware of this cause of assay interference.

Keywords: Thyroid hormones; analytes; clinical studies; immunoassay; laboratory methods; thyroid disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen-Antibody Complex / blood*
  • Antigen-Antibody Complex / chemistry
  • Chromatography, Gel
  • Congenital Hypothyroidism / blood*
  • Congenital Hypothyroidism / diagnosis
  • Congenital Hypothyroidism / immunology
  • False Positive Reactions
  • Humans
  • Immunoglobulin G / blood*
  • Immunoglobulin G / chemistry
  • Polyethylene Glycols / chemistry
  • Protein Binding
  • Thyroid Function Tests
  • Thyrotropin / blood*
  • Thyrotropin / chemistry


  • Antigen-Antibody Complex
  • Immunoglobulin G
  • Polyethylene Glycols
  • Thyrotropin