Signal transducer and activator of transcription 3 (STAT3) mutations underlying autosomal dominant hyper-IgE syndrome impair human CD8(+) T-cell memory formation and function

J Allergy Clin Immunol. 2013 Aug;132(2):400-11.e9. doi: 10.1016/j.jaci.2013.05.029. Epub 2013 Jul 4.


Background: The capacity of CD8(+) T cells to control infections and mediate antitumor immunity requires the development and survival of effector and memory cells. IL-21 has emerged as a potent inducer of CD8(+) T-cell effector function and memory development in mouse models of infectious disease. However, the role of IL-21 and associated signaling pathways in protective CD8(+) T-cell immunity in human subjects is unknown.

Objective: We sought to determine which signaling pathways mediate the effects of IL-21 on human CD8(+) T cells and whether defects in these pathways contribute to disease pathogenesis in patients with primary immunodeficiencies caused by mutations in components of the IL-21 signaling cascade.

Methods: Human primary immunodeficiencies resulting from monogenic mutations provide a unique opportunity to assess the requirement for particular molecules in regulating human lymphocyte function. Lymphocytes from patients with loss-of-function mutations in signal transducer and activator of transcription 1 (STAT1), STAT3, or IL-21 receptor (IL21R) were used to assess the respective roles of these genes in human CD8(+) T-cell differentiation in vivo and in vitro.

Results: Mutations in STAT3 and IL21R, but not STAT1, led to a decrease in multiple memory CD8(+) T-cell subsets in vivo, indicating that STAT3 signaling, possibly downstream of IL-21R, regulates the memory cell pool. Furthermore, STAT3 was important for inducing the lytic machinery in IL-21-stimulated naive CD8(+) T cells. However, this defect was overcome by T-cell receptor engagement.

Conclusion: The IL-21R/STAT3 pathway is required for many aspects of human CD8(+) T-cell behavior but in some cases can be compensated by other signals. This helps explain the relatively mild susceptibility to viral disease observed in STAT3- and IL-21R-deficient subjects.

Keywords: AD-HIES; Autosomal dominant hyper-IgE syndrome; B-cell lymphoma; BCL; CTV; CellTrace Violet; Central memory T; EOMES; Effector memory T; Effector memory T cells expressing CD45RA; Eomesodermin; IL-21; IL-21 receptor; IL-21R; LCMV; Lymphocytic choriomeningitis virus; PB; PID; Peripheral blood; Primary immunodeficiency; STAT; STAT1; STAT3; Signal transducer and activator of transcription; T(CM); T(EM); T(EMRA); T-cell receptor; TCR; differentiation; human CD8(+) T cells; memory.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD8-Positive T-Lymphocytes / cytology*
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / metabolism
  • Cell Differentiation* / genetics
  • Cell Differentiation* / immunology
  • Humans
  • Immunologic Memory*
  • Interleukins / genetics
  • Interleukins / immunology
  • Interleukins / metabolism
  • Job Syndrome / genetics*
  • Job Syndrome / immunology
  • Job Syndrome / pathology
  • Mutation*
  • Receptors, Interleukin-21 / genetics
  • Receptors, Interleukin-21 / immunology
  • Receptors, Interleukin-21 / metabolism
  • STAT3 Transcription Factor / genetics
  • STAT3 Transcription Factor / metabolism*


  • Interleukins
  • Receptors, Interleukin-21
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • interleukin-21