A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis

Nat Chem Biol. 2013 Aug;9(8):491-3. doi: 10.1038/nchembio.1289. Epub 2013 Jun 30.


We report a salvage pathway in Gram-negative bacteria that bypasses de novo biosynthesis of UDP N-acetylmuramic acid (UDP-MurNAc), the first committed peptidoglycan precursor, and thus provides a rationale for intrinsic fosfomycin resistance. The anomeric sugar kinase AmgK and the MurNAc α-1-phosphate uridylyl transferase MurU, defining this new cell wall sugar-recycling route in Pseudomonas putida, were characterized and engineered into Escherichia coli, channeling external MurNAc directly to peptidoglycan biosynthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Wall / chemistry
  • Cell Wall / metabolism*
  • Escherichia coli / enzymology
  • Escherichia coli / metabolism
  • Peptidoglycan / biosynthesis*
  • Peptidoglycan / chemistry
  • Pseudomonas putida / cytology
  • Pseudomonas putida / enzymology
  • Pseudomonas putida / metabolism*


  • Peptidoglycan