The UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine-phosphotransferase gene as a new selection marker for potato transformation

Hyun-Soon Kim; Mi-Sun Kim; Kyoung-A Hwang; Se-Won Park; Jae-Heung Jeon

doi:10.1271/bbb.130146

The UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine-phosphotransferase gene as a new selection marker for potato transformation

Biosci Biotechnol Biochem. 2013;77(7):1589-92. doi: 10.1271/bbb.130146. Epub 2013 Jul 7.

Authors

Hyun-Soon Kim¹, Mi-Sun Kim, Kyoung-A Hwang, Se-Won Park, Jae-Heung Jeon

Affiliation

¹ Green-Bio Research Center, KRIBB, Daejeon 305-806, Korea.

PMID: 23832343
DOI: 10.1271/bbb.130146

Abstract

Here we describe the generation of potato plants that constitutively overexpressed, UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine-phosphotransferase (GPT). Such transgenic plants can be formed in a medium with tunicamycin at 9.8 ± 0.28% efficiency, similar to the 9.4 ± 1.10 for the bialaphos resistance gene (Bar) gene. This study indicated that GPT transformation was very stable with high reproducibility, and that growth and tuber production in the GPT-transformed plants were stronger than in the wild-type plants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genetic Engineering / methods*
Genetic Markers / genetics
Genetic Vectors / genetics
Plants, Genetically Modified
Solanum tuberosum / genetics*
Transferases (Other Substituted Phosphate Groups) / genetics*
Transformation, Genetic*

Substances

Genetic Markers
Transferases (Other Substituted Phosphate Groups)
UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase