¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of a family 36 carbohydrate binding module of xylanase from Paenibacillus campinasensis

Biomol NMR Assign. 2014 Oct;8(2):303-6. doi: 10.1007/s12104-013-9505-3. Epub 2013 Jul 9.

Abstract

Paenibacillus campinasensis BL11 isolated from black liquor secretes multiple glycoside hydrolases (GHs) against all kinds of polysaccharides. GH consists of a catalytic module and non-catalytic carbohydrate-binding modules (CBMs), in which CBMs append to the catalytic module, mediating specific interactions with insoluble carbohydrates to promote the hydrolysis efficiency of the cognate enzyme. Endo-β-1,4-xylanase (XylX) is one of the GHs reveals high enzymatic activity in a wide range of pH and thermal endurance, suitable for bioconversion and bio-refinement applications. In this work, we report the resonance assignments of a family 36 CBM (characterized as CBM36) derived from XylX. Our investigations will facilitate molecular structure determination and molecular dynamics analysis of CBMs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carbohydrate Metabolism*
  • Endo-1,4-beta Xylanases / chemistry*
  • Endo-1,4-beta Xylanases / metabolism*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Paenibacillus / enzymology*

Substances

  • Endo-1,4-beta Xylanases