Determination of lipid hydroperoxides in low density lipoprotein from human plasma using high performance liquid chromatography with chemiluminescence detection

Biomed Chromatogr. 1990 May;4(3):131-4. doi: 10.1002/bmc.1130040312.


A high performance liquid chromatographic system with chemiluminescence detection (HPLC-CL) was used for determining phospholipid hydroperoxides in human plasma low density lipoprotein (LDL). This system involved separation of phospholipids from LDL-total lipids with normal phase silica gel HPLC and post-column detection of hydroperoxide-dependent chemiluminescence produced by luminol oxidation during the reaction of hydroperoxide with cytochrome c-haeme. By using HPLC-CL, we could detect phosphatidylcholine hydroperoxide (PCOOH) in human plasma LDL, and the LDL-PCOOH concentration was significantly higher in patients with atherosclerosis and hyperlipidemia than that of healthy volunteers. The LDL-PCOOH level was proportional to the plasma total cholesterol concentration.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Arteriosclerosis / blood
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Hyperlipidemias / blood
  • Lipid Peroxides / analysis
  • Lipid Peroxides / blood*
  • Lipoproteins, LDL / analysis
  • Lipoproteins, LDL / blood*
  • Luminescent Measurements*
  • Middle Aged


  • Lipid Peroxides
  • Lipoproteins, LDL