Peptidomic Identification of Serum Peptides Diagnosing Preeclampsia

PLoS One. 2013 Jun 19;8(6):e65571. doi: 10.1371/journal.pone.0065571. Print 2013.

Abstract

We sought to identify serological markers capable of diagnosing preeclampsia (PE). We performed serum peptide analysis (liquid chromatography mass spectrometry) of 62 unique samples from 31 PE patients and 31 healthy pregnant controls, with two-thirds used as a training set and the other third as a testing set. Differential serum peptide profiling identified 52 significant serum peptides, and a 19-peptide panel collectively discriminating PE in training sets (n = 21 PE, n = 21 control; specificity = 85.7% and sensitivity = 100%) and testing sets (n = 10 PE, n = 10 control; specificity = 80% and sensitivity = 100%). The panel peptides were derived from 6 different protein precursors: 13 from fibrinogen alpha (FGA), 1 from alpha-1-antitrypsin (A1AT), 1 from apolipoprotein L1 (APO-L1), 1 from inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), 2 from kininogen-1 (KNG1), and 1 from thymosin beta-4 (TMSB4). We concluded that serum peptides can accurately discriminate active PE. Measurement of a 19-peptide panel could be performed quickly and in a quantitative mass spectrometric platform available in clinical laboratories. This serum peptide panel quantification could provide clinical utility in predicting PE or differential diagnosis of PE from confounding chronic hypertension.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apolipoprotein L1
  • Apolipoproteins / chemistry
  • Biomarkers / blood
  • Blood Proteins / chemistry
  • Chromatography, Liquid / methods*
  • Female
  • Glycoproteins / chemistry
  • Humans
  • Kininogens / chemistry
  • Lipoproteins, HDL / chemistry
  • Peptide Fragments / blood*
  • Pre-Eclampsia / blood
  • Pre-Eclampsia / diagnosis*
  • Pregnancy
  • Proteinase Inhibitory Proteins, Secretory / chemistry
  • Sensitivity and Specificity
  • Tandem Mass Spectrometry / methods*
  • Thymosin / chemistry
  • Young Adult
  • alpha 1-Antitrypsin / chemistry

Substances

  • APOL1 protein, human
  • Apolipoprotein L1
  • Apolipoproteins
  • Biomarkers
  • Blood Proteins
  • Glycoproteins
  • ITIH4 protein, human
  • Kininogens
  • Lipoproteins, HDL
  • Peptide Fragments
  • Proteinase Inhibitory Proteins, Secretory
  • SERPINA1 protein, human
  • alpha 1-Antitrypsin
  • kininogen 1, human
  • thymosin beta(4)
  • Thymosin

Grant support

This work was supported in part by grants from the Spark Program at Stanford University, the March of Dimes, and the Lucile Packard Foundation for Children's Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.