The Effect of Thiamine and Thiamine Pyrophosphate on Oxidative Liver Damage Induced in Rats With Cisplatin

Biomed Res Int. 2013;2013:783809. doi: 10.1155/2013/783809. Epub 2013 Jun 6.

Abstract

The aim of this study was to investigate the effect of thiamine and thiamine pyrophosphate (TPP) on oxidative stress induced with cisplatin in liver tissue. Rats were divided into four groups; thiamine group (TG), TPP + cisplatin group (TPG), healthy animal group (HG), and cisplatin only group (CG). Oxidant and antioxidant parameters in liver tissue and AST, ALT, and LDH levels in rat sera were measured in all groups. Malondialdehyde levels in the CG, TG, TPG, and HG groups were 11 ± 1.4, 9 ± 0.5, 3 ± 0.5, and 2.2 ± 0.48 μ mol/g protein, respectively. Total glutathione levels were 2 ± 0.7, 2.8 ± 0.4, 7 ± 0.8, and 9 ± 0.6 nmol/g protein, respectively. Levels of 8-OH/Gua, a product of DNA damage, were 2.7 ± 0.4 pmol/L, 2.5 ± 0.5, 1.1 ± 0.3, and 0.9 ± 0.3 pmol/L, respectively. A statistically significant difference was determined in oxidant/antioxidant parameters and AST, ALT, and LDH levels between the TPG and CG groups (P < 0.05). No significant difference was determined between the TG and CG groups (P > 0.05). In conclusion, cisplatin causes oxidative damage in liver tissue. TPP seems to have a preventive effect on oxidative stress in the liver caused by cisplatin.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Aspartate Aminotransferases / metabolism
  • Chemical and Drug Induced Liver Injury / drug therapy
  • Chemical and Drug Induced Liver Injury / metabolism*
  • Chemical and Drug Induced Liver Injury / pathology
  • Cisplatin / administration & dosage
  • Cisplatin / adverse effects
  • DNA Damage / drug effects
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Humans
  • Lipid Peroxidation / drug effects
  • Male
  • Oxidative Stress / drug effects*
  • Protective Agents / administration & dosage*
  • Rats
  • Superoxide Dismutase / metabolism
  • Thiamine / administration & dosage*
  • Thiamine Pyrophosphate / administration & dosage*

Substances

  • Antioxidants
  • Protective Agents
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Aspartate Aminotransferases
  • Glutathione
  • Cisplatin
  • Thiamine Pyrophosphate
  • Thiamine