Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library

FEBS Lett. 1990 Jul 30;268(1):63-8. doi: 10.1016/0014-5793(90)80973-m.


A full-length K+ channel cDNA (RHK1) was isolated from a rat cardiac library using the polymerase chain reaction (PCR) method and degenerate oligonucleotide primers derived from K+ channel sequences conserved between Drosophila Shaker H4 and mouse brain MBK1. Although RHK1 was isolated from heart, its expression was found in both heart and brain. The RHK1-encoded protein, when expressed in Xenopus oocytes, gated a 4-aminopyridine (4-AP)-sensitive transient outward current. This current is similar to the transient outward current measured in rat ventricular myocytes with respect to voltage-dependence of activation and inactivation, time course of activation and inactivation, and pharmacology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Blotting, Southern
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Gene Library
  • Membrane Potentials
  • Molecular Sequence Data
  • Myocardium
  • Potassium Channels* / physiology
  • RNA, Messenger / genetics
  • Rats
  • Xenopus laevis


  • Potassium Channels
  • RNA, Messenger
  • DNA