Effect of copaiba oil in hepatic damage induced by acetaminophen in rats

Acta Cir Bras. 2013 Jul;28(7):526-30. doi: 10.1590/s0102-86502013000700008.

Abstract

Purpose: To investigate the effects of copaiba oil on the hepatic damage induced by paracetamol.

Methods: Thirty six rats were distributed into six study groups (N=6): control group, that didn't receive the acetaminophen; Acetaminophen Group, that only received the acetaminophen; Prophylactic Copaiba Group 1, that received copaiba oil two hours before the acetaminophen; Prophylactic Copaiba Group 7, that received copaiba oil seven days, once by day, before the acetaminophen; Therapy Copaiba Group, that received the copaiba oil two hours afther the acetaminophen; and N-Acetyl-Cysteine Group, , that received the N-Acetyl-Cysteine two hours afther the acetaminophen. Euthanasia was performed after 24 hours. The serum levels of AST, ALT, alkaline phosphatase, [formula see text] GT, total bilirubin, direct bilirubin and indirect bilirubin and histological analisis were analized.

Results: The prophylactic copaiba group 7, therapy copaiba group and N-Acetyl-Cysteine Group showed amounts of AST and ALT similar to the control group; and the prophylactic copaiba group 1 showed similar levels to the acetaminophen group. There was no significant difference between the groups regarding the amount of alkaline phosphatase and [formula see text] GT (p>0.05). The therapy copaiba group showed the highest levels of bilirubin and was statistically different from the other groups (p<0.01) and this increased the costs of direct bilirubin. Regarding histopathology, the oil of copaiba administered prophylactic or therapeutic form for 7 days could decrease the amount of necrosis and inflammatory infiltrate.

Conclusion: Copaiba oil administered prophylactically for seven days, and therapeutic could reduce liver damage caused by paracetamol similarly N-Acetyl-Cysteine, however, when treated with copaiba therapeutically showed increases in bilirubin, costs increasing fraction indirect.

MeSH terms

  • Acetaminophen / adverse effects*
  • Analgesics, Non-Narcotic / adverse effects*
  • Animals
  • Bilirubin / blood
  • Chemical and Drug Induced Liver Injury / drug therapy*
  • Chemical and Drug Induced Liver Injury / pathology
  • Fabaceae / chemistry*
  • Male
  • Plant Oils / therapeutic use*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Reproducibility of Results
  • Time Factors
  • Treatment Outcome

Substances

  • Analgesics, Non-Narcotic
  • Plant Oils
  • Acetaminophen
  • Bilirubin