The chemoprotective effects of L-carnitine against genotoxicity induced by diazinon in rat blood lymphocyte

Toxicol Ind Health. 2015 Dec;31(12):1334-40. doi: 10.1177/0748233713491811. Epub 2013 Jul 10.

Abstract

The purpose of this study was to assess the preventive effects of L-carnitine (LC) against DNA damage induced by diazinon (DZN) in rat blood lymphocytes. Animals were concurrently administered intraperitoneally with DZN in proper solvent (20 mg/kg body weight (b.w.)) and LC at three different doses (50, 100, and 150 mg/kg b.w.) for 30 consecutive days. The positive control group received DZN at the same dose without LC. Twenty-four hour after last injection, 0.5 ml blood of each rat was received and cultured in culture medium for 44 h. The lymphocyte cultures were mitogenically stimulated with cytochalasin B for the evaluation of the number of micronuclei (MNs) in cytokinesis-blocked binucleated cells. Incubation of lymphocytes with DZN induced additional genotoxicity and was shown by increase in MNs frequency in rat lymphocytes. LC at all doses had a protective effect and significantly reduced the MNs frequency in cultured lymphocytes (p < 0.0001-p < 0.05). The maximum effect was observed at 150 mg/kg that reduced the frequency of MN from 12.78 ± 0.24% for DZN group to 5.61 ± 0.17%. Our study revealed that LC has a potent antigenotoxic effect against DZN-induced toxicity in rats, which may be due to the scavenging of free radicals and increased antioxidant status. Since LC is a natural compound and is being safe, it is recommended as a daily supplement for body defense against side effects induced by chemical hazardous agents.

Keywords: Chemoprotective; diazinon; genotoxicity; l-carnitine; micronucleus assay; rat blood lymphocyte.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticarcinogenic Agents / administration & dosage
  • Anticarcinogenic Agents / therapeutic use
  • Antioxidants / administration & dosage
  • Antioxidants / therapeutic use
  • Carnitine / administration & dosage
  • Carnitine / therapeutic use*
  • Cells, Cultured
  • DNA Damage / drug effects*
  • DNA Repair / drug effects
  • Diazinon / administration & dosage
  • Diazinon / antagonists & inhibitors*
  • Diazinon / toxicity
  • Dose-Response Relationship, Drug
  • Injections, Intraperitoneal
  • Insecticides / administration & dosage
  • Insecticides / antagonists & inhibitors*
  • Insecticides / toxicity
  • Lymphocytes / drug effects*
  • Lymphocytes / pathology
  • Male
  • Micronuclei, Chromosome-Defective / chemically induced
  • Micronuclei, Chromosome-Defective / drug effects
  • Micronucleus Tests
  • Mutagens / administration & dosage
  • Mutagens / chemistry*
  • Mutagens / toxicity
  • Protective Agents / administration & dosage
  • Protective Agents / therapeutic use*
  • Random Allocation
  • Rats, Wistar

Substances

  • Anticarcinogenic Agents
  • Antioxidants
  • Insecticides
  • Mutagens
  • Protective Agents
  • Carnitine
  • Diazinon