Development and characterization of a chemically defined food for Drosophila

PLoS One. 2013 Jul 2;8(7):e67308. doi: 10.1371/journal.pone.0067308. Print 2013.

Abstract

Diet can affect a spectrum of biological processes ranging from behavior to cellular metabolism. Yet, the precise role of an individual dietary constituent can be a difficult variable to isolate experimentally. A chemically defined food (CDF) permits the systematic evaluation of individual macro- and micronutrients. In addition, CDF facilitates the direct comparison of data obtained independently from different laboratories. Here, we report the development and characterization of a CDF for Drosophila. We show that CDF can support the long-term culture of laboratory strains and demonstrate that this formulation has utility in isolating macronutrient from caloric density requirements in studies of development, longevity and reproduction.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Drosophila melanogaster / physiology*
  • Female
  • Food, Formulated / analysis*
  • Larva / physiology*
  • Longevity
  • Male
  • Micronutrients / chemistry
  • Nutritional Requirements / physiology*
  • Reproduction / physiology*
  • Zygote / physiology*

Substances

  • Micronutrients