Colonic mucosa-associated diffusely adherent afaC+ Escherichia coli expressing lpfA and pks are increased in inflammatory bowel disease and colon cancer

Gut. 2014 May;63(5):761-70. doi: 10.1136/gutjnl-2013-304739. Epub 2013 Jul 11.

Abstract

Objective: Colonic mucosa-associated Escherichia coli are increased in Crohn's disease (CD) and colorectal cancer (CRC). They variously haemagglutinate, invade epithelial cell lines, replicate within macrophages, translocate across M (microfold) cells and damage DNA. We investigated genes responsible for these effects and their co-association in colonic mucosal isolates.

Design: A fosmid library yielding 968 clones was prepared in E coli EPI300-T1 using DNA from a haemagglutinating CRC isolate, and resulting haemagglutinating clones were 454-pyrosequenced. PCR screening was performed on 281 colonic E coli isolates from inflammatory bowel disease (IBD) (35 patients), CRC (21) and controls (24; sporadic polyps or irritable bowel syndrome).

Results: 454-Pyrosequencing of fosmids from the haemagglutinating clones (n=8) identified the afimbrial adhesin afa-1 operon. Transfection of afa-1 into E coli K-12 predictably conferred diffuse adherence plus invasion of HEp-2 and I-407 epithelial cells, and upregulation of vascular endothelial growth factor. E coli expressing afaC were common in CRC (14/21, p=0.0009) and CD (9/14, p=0.005) but not ulcerative colitis (UC; 8/21) compared with controls (4/24). E coli expressing both afaC and lpfA (relevant to M-cell translocation) were common in CD (8/14, p=0.0019) and CRC (14/21, p=0.0001), but not UC (6/21) compared with controls (2/24). E coli expressing both afaC and pks (genotoxic) were common in CRC (11/21, p=0.0015) and UC (8/21, p=0.022), but not CD (4/14) compared with controls (2/24). All isolates expressed dsbA and htrA relevant to intra-macrophage replication, and 242/281 expressed fimH encoding type-1 fimbrial adhesin.

Conclusions: IBD and CRC commonly have colonic mucosal E coli that express genes that confer properties relevant to pathogenesis including M-cell translocation, angiogenesis and genotoxicity.

Keywords: BACTERIAL ADHERENCE; BACTERIAL INTERACTIONS; BACTERIAL PATHOGENESIS; E. COLI; GUT INFLAMMATION.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Escherichia coli / metabolism
  • Base Sequence
  • Biomarkers / metabolism
  • Caco-2 Cells
  • Case-Control Studies
  • Cell Line
  • Colon / microbiology*
  • Colonic Neoplasms / microbiology*
  • DNA, Bacterial / analysis
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Escherichia coli / metabolism*
  • Escherichia coli / pathogenicity
  • Escherichia coli Proteins / metabolism*
  • Fimbriae Proteins / metabolism*
  • Hemagglutinins / metabolism
  • Humans
  • Inflammatory Bowel Diseases / microbiology*
  • Intestinal Mucosa / microbiology*
  • Molecular Sequence Data
  • Polyketide Synthases / metabolism
  • Real-Time Polymerase Chain Reaction
  • Sequence Analysis, DNA

Substances

  • Adhesins, Escherichia coli
  • Biomarkers
  • DNA, Bacterial
  • Escherichia coli Proteins
  • Hemagglutinins
  • LpfA protein, E coli
  • Fimbriae Proteins
  • Polyketide Synthases

Associated data

  • GENBANK/JN688153